The effect of laser on growth of 2 Type from Bacteria G ve (Escherichia coli) and G+ve (Staphylococcus aureus) was studied, by radiated the samples in a different periods (5,10) min. S. aureus was more effected from E.coli , also the sensitivity to Antibiotics was studied befor and after radiated to laser diode power(5mw) and some different s was observed to the sensitivity of antibiotics from this bacteria .